3BRH
Protein Tyrosine Phosphatase PTPN-22 (Lyp) bound to the mono-Phosphorylated Lck active site peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.850, 48.510, 119.970 |
Unit cell angles | 90.00, 103.49, 90.00 |
Refinement procedure
Resolution | 29.591 - 2.200 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.26430 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.965 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.259 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 34892 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 96.2 | 97.46 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Bicine, 20% PEG6000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |