3BPU
Crystal structure of the 3rd PDZ domain of human membrane associated guanylate kinase, C677S and C709S double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97912 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 32.472, 61.578, 80.113 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.740 - 1.600 |
R-factor | 0.20418 |
Rwork | 0.202 |
R-free | 0.25112 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1UJV 1g9o |
RMSD bond length | 0.016 |
RMSD bond angle | 1.597 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.060 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.074 | 0.898 |
Number of reflections | 10446 | |
<I/σ(I)> | 17.1 | 2.2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.05M Zn acetate pH 6.2, 30% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |