3BNU
Crystal structure of polyamine oxidase FMS1 from Saccharomyces cerevisiae in complex with bis-(3S,3'S)-methylated spermine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9779 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.195, 103.003, 77.518 |
Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.2175 |
Rwork | 0.217 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.061 | 0.272 |
Number of reflections | 61584 | |
Completeness [%] | 95.6 | 73.2 |
Redundancy | 3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 18% PEG 4000, 0.2M Li2SO4, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |