3BNF
W. succinogenes NrfA Sulfite Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0600 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 119.500, 119.500, 186.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.310 - 1.700 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.27954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fs7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.778 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 73146 | |
| <I/σ(I)> | 16.4 | 2 |
| Completeness [%] | 98.4 | 96.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | PEG 4000, ammonium sulfate, yttrium chloride, acetate buffer, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
