3BLW
Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 113.157, 116.346, 163.620 |
Unit cell angles | 98.96, 110.23, 106.63 |
Refinement procedure
Resolution | 49.383 - 4.300 |
R-factor | 0.2758 |
Rwork | 0.274 |
R-free | 0.31060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3blv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.450 |
High resolution limit [Å] | 4.300 | 4.300 |
Number of reflections | 44752 | |
<I/σ(I)> | 7.9 | 2.4 |
Completeness [%] | 90.1 | 66.7 |
Redundancy | 3.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 18% polyethylene glycol 3350, 0.2 M potassium sulfate, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |