3BLW
Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.157, 116.346, 163.620 |
| Unit cell angles | 98.96, 110.23, 106.63 |
Refinement procedure
| Resolution | 49.383 - 4.300 |
| R-factor | 0.2758 |
| Rwork | 0.274 |
| R-free | 0.31060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3blv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.450 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Number of reflections | 44752 | |
| <I/σ(I)> | 7.9 | 2.4 |
| Completeness [%] | 90.1 | 66.7 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 18% polyethylene glycol 3350, 0.2 M potassium sulfate, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
