3BLH
Crystal Structure of Human CDK9/cyclinT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9756 |
Spacegroup name | H 3 |
Unit cell lengths | 172.920, 172.920, 95.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.726 - 2.480 |
Rwork | 0.179 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1QMZ FOR CHAIN A PDB ENTRY 2PK2 FOR CHAIN B |
RMSD bond length | 0.005 |
RMSD bond angle | 0.646 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.460 | 48.740 | 2.610 |
High resolution limit [Å] | 2.480 | 7.840 | 2.480 |
Rmerge | 0.063 | 0.044 | 0.472 |
Total number of observations | 2350 | 15467 | |
Number of reflections | 37491 | ||
<I/σ(I)> | 7.8 | 14.1 | 1.6 |
Completeness [%] | 99.1 | 76.5 | 100 |
Redundancy | 2.8 | 2.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris, 20% PEG 1000, 0.5M NaCl, 4mM TCEP, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |