3BK3
Crystal structure of the complex of BMP-2 and the first Von Willebrand domain type C of Crossveinless-2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9799, 0.9796, 0.9079 |
Spacegroup name | I 41 |
Unit cell lengths | 83.750, 83.750, 139.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24500 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.603 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 13191 | |
<I/σ(I)> | 8.9 | 3.3 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2M ammonium phosphate, 0.1M Tris pH 7.5, 8% glycerol, 5% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 294K |