3BK1
Crystal Structure Analysis of RNase J
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 97.830, 117.620, 230.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.750 - 2.330 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 2.400 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.060 | 0.171 |
| Number of reflections | 28236 | |
| <I/σ(I)> | 30.53 | 10.22 |
| Completeness [%] | 98.7 | 88.5 |
| Redundancy | 13.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2-4% PEG 4K, 0.02M ammonium sulfate, 0.1M Na-MES, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
