3BJU
Crystal Structure of tetrameric form of human lysyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 152.013, 152.013, 108.838 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.310 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e24 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.313 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.530 |
| Number of reflections | 160180 | |
| <I/σ(I)> | 5.7 | 0.87 |
| Completeness [%] | 98.0 | 98.2 |
| Redundancy | 1.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.75 | 277 | 12% PEG8000, 16% Glycerol, 0.08M Na Cacodylate, 0.16M Ca(OAc)2, pH 5.75, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
