3BGV
Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792996 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.787, 99.672, 167.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.510 - 2.300 |
R-factor | 0.22486 |
Rwork | 0.221 |
R-free | 0.29448 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ri1 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.697 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 58716 | |
<I/σ(I)> | 27.9 | 3.35 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 17.6 | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 20% PEG 3350, 0.2 M KCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |