3BCC
STIGMATELLIN AND ANTIMYCIN BOUND CYTOCHROME BC1 COMPLEX FROM CHICKEN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-29 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 173.180, 179.730, 238.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.700 |
| R-factor | 0.289 |
| Rwork | 0.289 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT USING NATIVE STRUCTURE SOLVED BY THE SAME AUTHOR |
| Starting model (for MR) | 1bcc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TFFC |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.630 | |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.156 | 0.383 |
| Number of reflections | 80888 | |
| <I/σ(I)> | 6.6 | 1.6 |
| Completeness [%] | 85.8 | 75.7 |
| Redundancy | 2.84 | 1.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 | 20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, INHIBITOR WAS ADDED FROM ETHANOLIC SOLUTION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | KMES | 60 (mM) | pH6.7 |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | K-MOPS 7.5 | 20 (mM) | |
| 4 | 1 | drop | n-octyl-beta-D-glucopyranoside | 20 (g/l) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | reservoir | glycerol | 30 (%) |
