3BCA
Crystal structure of mouse selenocysteine synthase, sodium iodide soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.222, 138.371, 141.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.18526 |
| Rwork | 0.183 |
| R-free | 0.23566 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.229 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 52394 | |
| <I/σ(I)> | 13.4 | 3.8 |
| Completeness [%] | 98.3 | 97 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 11 % (v/v) Ethylene glycol, soaked in 0.5 M NaI, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
