3BAP

Crystal Structure of the 25 kDa Subunit of Human Cleavage Factor Im

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97923, 0.97939, 0.97166
Spacegroup name	P 31 2 1
Unit cell lengths	80.115, 80.115, 72.209
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.226
Rwork	0.226
R-free	0.24400
RMSD bond length	0.006
RMSD bond angle	1.339
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.890
High resolution limit [Å]	1.820	1.820
Rmerge	0.086	0.052
Number of reflections	22805
<I/σ(I)>	16.09	2.2
Completeness [%]	98.1
Redundancy	6.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	285	25% PEG 3350, 0.2M Magnesium chloride, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K