3BAH
Crystal structure of K112N mutant of Human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-12-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.407, 96.317, 108.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.700 - 1.650 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.700 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.066 | 0.411 |
Number of reflections | 43314 | |
<I/σ(I)> | 45.57 | 3.63 |
Completeness [%] | 90.4 | 99.9 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 2.9M Na-formate, 1.0M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |