3B9Q
The crystal structure of cpFtsY from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 87.897, 84.150, 99.374 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.27944 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fts |
RMSD bond length | 0.017 |
RMSD bond angle | 1.440 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.098 | 0.405 |
Number of reflections | 36935 | |
<I/σ(I)> | 14 | 2.9 |
Completeness [%] | 98.9 | 96.2 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |