3B8E
Crystal structure of the sodium-potassium pump
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.078, 1.073, 0.81 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.930, 261.500, 334.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| Rwork | 0.277 |
| R-free | 0.31290 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.518 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.258 | 1.200 |
| Number of reflections | 77431 | |
| <I/σ(I)> | 11.24 | 2.56 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.8 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 14% PEG 2000mme, 0.2M Choline Chloride, 4% Glycerol, 4% MPD, 0.04M DTT, 0.1-04% beta-DDM, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
