3B8A
Crystal structure of yeast hexokinase PI in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.120, 78.870, 144.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.920 - 2.950 |
| Rwork | 0.192 |
| R-free | 0.24400 |
| Starting model (for MR) | 1ig8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.920 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 14357 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 92.9 | 94.44 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 277 | 30% PEG 4000, 0.1M phosphate buffer, 10 mg/ml protein concentration, pH 6.5, hanging drop, temperature 277K |
