3B82
Structure of the eEF2-ExoA(E546H)-NAD+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 327.570, 68.460, 189.990 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.350 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25700 |
| Starting model (for MR) | 1zm9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.547 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.144 | 0.698 |
| Number of reflections | 169084 | 8495 |
| <I/σ(I)> | 9.07 | 1.6 |
| Completeness [%] | 98.4 | 81.9 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
