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3B7Z

Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylcholine or Phosphatidylinositol

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2006-06-14
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1
Spacegroup nameP 21 21 21
Unit cell lengths49.354, 72.931, 97.594
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.560 - 2.030
Rwork0.201
R-free0.23800
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle1.200
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.100
High resolution limit [Å]2.0302.030
Rmerge0.0860.289
Number of reflections22693
<I/σ(I)>14.2
Completeness [%]95.893.2
Redundancy4.13.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.629515-25% PEG 3350, 5% glycerol, and either 100 mM ammonium sulfate or 100mM potassium phosphate, 100 mM ammonium acetate, pH 4.6. Crystals were cryoprotected in 30% PEG 3350, 30% glycerol, and 200 mM ammonium sulfate, 100 mM ammonium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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