3B7D
Crystal structure of the GLUR2 ligand binding core (HS1S2J) in complex with CNQX at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.582, 97.631, 100.970 |
| Unit cell angles | 81.85, 89.08, 77.89 |
Refinement procedure
| Resolution | 39.900 - 2.500 |
| R-factor | 0.25598 |
| Rwork | 0.255 |
| R-free | 0.28313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.426 | |
| Number of reflections | 92349 | |
| <I/σ(I)> | 1.87 | |
| Completeness [%] | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 40 mM Sodium citrate, 10 mM Ammonium sulfate, 1% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
