3B71
CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97982 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.071, 220.472, 97.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.820 |
| R-factor | 0.24195 |
| Rwork | 0.241 |
| R-free | 0.30712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k05 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.852 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.970 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.143 | 0.699 |
| Number of reflections | 21922 | |
| <I/σ(I)> | 9.2 | 1.1 |
| Completeness [%] | 93.9 | 69.9 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 3.9 M NaCl, 2.5 % glycerol, 100 mM Hepes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
