3B6V

Crystal structure of the motor domain of human kinesin family member 3C in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	135.739, 47.588, 116.951
Unit cell angles	90.00, 103.16, 90.00

Refinement procedure

Resolution	30.000 - 2.700
Rwork	0.202
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ii6
RMSD bond length	0.014
RMSD bond angle	1.229
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.126	0.055	0.470
Number of reflections	20103
<I/σ(I)>	8.1
Completeness [%]	97.2	97.4	82.4
Redundancy	3.4	3.4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	30% PEG MME550, 0.1M Ammonium sulfate, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 293K