3B6M
WrbA from Escherichia coli, second crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.800, 62.800, 201.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.880 - 1.850 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.22344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b6i |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.671 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 35588 | |
| <I/σ(I)> | 10.3 | 2.6 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 6.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 15-30 % Polyethylene Glycol, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 100K, temperature 293K |
