3B54
Saccharomyces cerevisiae nucleoside diphosphate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-27 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 185.920, 185.920, 185.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.23221 |
| Rwork | 0.231 |
| R-free | 0.26081 |
| Structure solution method | MIR |
| Starting model (for MR) | 1nue |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.939 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.112 | 0.700 |
| Number of reflections | 10547 | |
| <I/σ(I)> | 5.6122 | 1.1 |
| Completeness [%] | 98.2 | 93.35 |
| Redundancy | 6.41 | 4.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 291 | 13% isopropanol, 19% TBA(tert-butyl alcohol), 0.1M Na Citrate pH 5.6, VAPOR DIFFUSION, temperature 291K |
