3B4X
Crystal Structure Analysis of Sulfolobus tokodaii strain7 cytochrom P450
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.286, 55.168, 51.415 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 39.580 - 1.940 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ue8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.580 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.056 | 0.277 |
| Number of reflections | 25507 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 90.4 | 67 |
| Redundancy | 4.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 20% PEG 4000, 0.2M ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
