1UE8

Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii

Summary for 1UE8

• Entry DOI: 10.2210/pdb1ue8/pdb
• Descriptor: 367aa long hypothetical cytochrome P450, CHLORIDE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• Functional Keywords: alpha-domain, structural genomics, unknown function
• Biological source: Sulfolobus tokodaii
• Cellular location: Cytoplasm (By similarity): Q972I2
• Total number of polymer chains: 1
• Total formula weight: 43066.75

Authors

Nakamura, N.,Kamitori, S.,Ohno, H. (deposition date: 2003-05-09, release date: 2004-07-13, Last modification date: 2023-12-27)

Primary citation

Oku, Y.,Ohtaki, A.,Kamitori, S.,Nakamura, N.,Yohda, M.,Ohno, H.,Kawarabayasi, Y.
Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7
J.Inorg.Biochem., 98:1194-1199, 2004

PubMed Abstract: Cytochrome P450 from thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (P450st) has been expressed in Escherichia coli and purified at high homogeneity. P450st was crystallized in an orthorhombic system with the space group P2(1)2(1)2(1) and cell dimensions of a=53.6 A, b=55.1 A, and c=130.9 A, and the structure was determined at a 3.0 A resolution. The final R-factor was 0.194 (Rfree=0.235). Structural comparison with cytochrome P450 from S. solfataricus (CYP119) suggests that the region composed of the F to G helices and the Cl- binding site is responsible for the affinity for a ligand coordinating heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide (DDAB) film on a plastic formed carbon (PFC) electrode has also been demonstrated. A quasi-reversible redox response has been observed even at elevated temperatures of up to 80 degrees C.

PubMed: 15219985
DOI: 10.1016/j.jinorgbio.2004.05.002

Experimental method

X-RAY DIFFRACTION (3 Å)