3B4T

Crystal structure of Mycobacterium tuberculosis RNase PH, the Mycobacterium tuberculosis Structural Genomics Consortium target Rv1340

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-02
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	73.529, 152.406, 83.512
Unit cell angles	90.00, 109.46, 90.00

Refinement procedure

Resolution	39.690 - 2.100
Rwork	0.194
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r6m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	DM
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.811	2.180
High resolution limit [Å]	2.090	2.090
Rmerge	0.036	0.422
Number of reflections	399198
<I/σ(I)>	22.2	2.58
Completeness [%]	100.0	99.7
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	22.5% PEG 2000, 0.1M MOPS pH 7.5, 0.085M NaH2PO4, 0.085M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K, pH 7.50