3B34
Crystal structure of E. coli Aminopeptidase N in complex with Phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.582, 120.582, 170.761 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.300 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.19416 |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.490 | |
Number of reflections | 346171 | |
<I/σ(I)> | 8.2 | 1.18 |
Completeness [%] | 99.2 | 89.9 |
Redundancy | 8.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M Sodium Malonate, pH=7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |