3B20
Crystal structure of Glyceraldehyde-3-Phosphate Dehydrogenase complexed with NADfrom Synechococcus elongatus"
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.589, 79.847, 206.794 |
| Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
| Resolution | 45.433 - 2.398 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.398 | 2.400 |
| Rmerge | 0.367 | |
| Number of reflections | 94315 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40% saturated ammonium sulfate, 0.1M citrate buffer pH 5.2, 0.2M potassium sodium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
