3B1K
Crystal structure of Glyceraldehyde-3-Phosphate Dehydrogenase complexed with CP12 in the absence of copper from Synechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Collection date | 2009-11-16 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.042, 146.887, 161.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.062 - 3.302 |
| R-factor | 0.2576 |
| Rwork | 0.252 |
| R-free | 0.36280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d2i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.147 | 0.350 |
| Number of reflections | 22942 | |
| Completeness [%] | 89.0 | 85.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20%(v/v) PEG3350, 0.2M magnesium acetate., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
