3B0V
tRNA-dihydrouridine synthase from Thermus thermophilus in complex with tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97881 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.890, 118.890, 319.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.600 - 3.510 |
| Rwork | 0.300 |
| R-free | 0.32000 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | AutoSol |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.605 | 48.605 | 3.700 |
| High resolution limit [Å] | 3.510 | 11.110 | 3.510 |
| Rmerge | 0.061 | 0.531 | |
| Rmeas | 0.065 | 0.555 | |
| Rpim | 0.019 | 0.158 | |
| Total number of observations | 10533 | 47935 | |
| Number of reflections | 29454 | ||
| <I/σ(I)> | 17.1 | 34.2 | 4.6 |
| Completeness [%] | 99.5 | 98.6 | 97.1 |
| Redundancy | 13.2 | 9.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-HCl, ammonium sulfate, glycerol, pH 7-8, vapor diffusion, sitting drop, temperature 293K |
