3B0V

tRNA-dihydrouridine synthase from Thermus thermophilus in complex with tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97881
Spacegroup name	P 41 21 2
Unit cell lengths	118.890, 118.890, 319.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 3.510
Rwork	0.300
R-free	0.32000
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	AutoSol
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.605	48.605	3.700
High resolution limit [Å]	3.510	11.110	3.510
Rmerge		0.061	0.531
Rmeas		0.065	0.555
Rpim		0.019	0.158
Total number of observations		10533	47935
Number of reflections	29454
<I/σ(I)>	17.1	34.2	4.6
Completeness [%]	99.5	98.6	97.1
Redundancy	13.2	9.8	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-HCl, ammonium sulfate, glycerol, pH 7-8, vapor diffusion, sitting drop, temperature 293K