3B0P

tRNA-dihydrouridine synthase from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	42.010, 60.190, 67.000
Unit cell angles	76.22, 88.67, 70.67

Refinement procedure

Resolution	19.775 - 1.700
R-factor	0.1701
Rwork	0.168
R-free	0.20240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (dev_777)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.700
Number of reflections	63578
<I/σ(I)>	17.94
Completeness [%]	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, MgCl2, PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K