3AYC
Crystal structure of galectin-3 CRD domian complexed with GM1 pentasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2010-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.540, 51.640, 59.670 |
| Unit cell angles | 64.44, 85.23, 81.04 |
Refinement procedure
| Resolution | 28.400 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nn8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.280 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.083 | 0.137 |
| Number of reflections | 31267 | |
| <I/σ(I)> | 10.9 | 6.8 |
| Completeness [%] | 97.5 | 96.3 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2.0 A.S., 0.1M Bis-Tris, 5mM beta-Mercaptoethanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
