3AY0
Crystal structure of Methanocaldococcus jannaschii Trm5 in complex with adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.490, 67.127, 92.239 |
| Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.050 |
| Rwork | 0.214 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yx1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 14439 | |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.02M HEPES, 0.175M sodium thiocyanate, 8% PEG 3350, 5% PEGMME 550, 0.002M zinc sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
