3AXK

Structure of rice Rubisco in complex with NADP(H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 4
Unit cell lengths	110.246, 110.246, 199.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.630 - 1.900
R-factor	0.169
Rwork	0.169
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	39.630
High resolution limit [Å]	1.900
Rmerge	0.070
Number of reflections	89311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	75mM HEPES-KOH (pH 7.75), 9% PEG4000, 25% glycerol, VAPOR DIFFUSION, temperature 293K