3AV5

Crystal structure of mouse DNA methyltransferase 1 with AdoHcy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-10
Detector	RAYONIX MX225HE
Wavelength(s)	0.900
Spacegroup name	P 21 21 2
Unit cell lengths	134.993, 96.920, 130.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.660 - 3.250
R-factor	0.1876
Rwork	0.183
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av4
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	3.360
High resolution limit [Å]	3.250	8.960	3.300
Rmerge	0.102	0.032	0.344
Number of reflections	26418
<I/σ(I)>	8.5
Completeness [%]	95.7	89.2	97.8
Redundancy	5.1	4.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	297	PEG 3350, NaCl, TCEP, pH 9.0, vapor diffusion, temperature 297K