3ATR
Geranylgeranyl Reductase (GGR) from Sulfolobus acidocaldarius co-crystallized with its ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.984, 82.054, 105.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.180 - 1.800 |
| R-factor | 0.17836 |
| Rwork | 0.177 |
| R-free | 0.20957 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.334 |
| Number of reflections | 49575 | |
| <I/σ(I)> | 37.7 | |
| Completeness [%] | 96.6 | 74.8 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.6 | 293 | 3.5-4.5M Sodium Formate, 0.1M Sodium Acetate Buffer, pH 4.6, temperature 293K, LIQUID DIFFUSION |
