3AQF

Crystal structure of the human CRLR/RAMP2 extracellular complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790, 0.9793, 0.9640
Spacegroup name	P 41 21 2
Unit cell lengths	55.424, 55.424, 119.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.620 - 2.600
Rwork	0.222
R-free	0.28210
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	6157
<I/σ(I)>	20.3	5.9
Completeness [%]	99.1	91.8
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M bis-Tris, 30 % (v/v) PEG MME 550, 0.05M Calcium Chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K