3AOE

Crystal structure of hetero-hexameric glutamate dehydrogenase from Thermus thermophilus (Leu bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-05-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	121.986, 146.093, 158.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.220 - 2.600
R-factor	0.19594
Rwork	0.193
R-free	0.25961
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b26
RMSD bond length	0.012
RMSD bond angle	1.464
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.220	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	87634
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M sodium citrate tribasic (pH5.6), 1.0M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 293K