3ANZ
Crystal Structure of alpha-hemolysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.821, 293.877, 170.545 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 19.984 - 2.303 |
| R-factor | 0.2045 |
| Rwork | 0.193 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ahl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.911 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.085 | 0.066 | 0.399 |
| Number of reflections | 439066 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 99.1 | 95.3 | 99 |
| Redundancy | 3.4 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES, Ammonium acetate, MPD, pH 7.5, vapor diffusion, sitting drop, temperature 293K |
