3AN2
The structure of the centromeric nucleosome containing CENP-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.840, 83.290, 176.830 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.271 |
| Rwork | 0.271 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
| Number of reflections | 21270 | ||
| <I/σ(I)> | 8.3 | 41.8 | 3.2 |
| Completeness [%] | 95.0 | 98.1 | 87 |
| Redundancy | 3.5 | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
