3AKH
Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-06-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.237, 91.341, 135.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 1.700 |
| R-factor | 0.18963 |
| Rwork | 0.188 |
| R-free | 0.21972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3akf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.257 |
| Number of reflections | 56484 | |
| <I/σ(I)> | 53.7 | 9.53 |
| Completeness [%] | 97.9 | 97 |
| Redundancy | 14.6 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8M sodium citrate, 0.2M sodium chloride, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
