3AKC
Crystal structure of CMP kinase in complex with CDP and ADP from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-07-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 62.206, 62.206, 110.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.650 - 1.650 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cmk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.640 | 1.650 |
| Rmerge | 0.067 | 0.287 |
| Number of reflections | 28624 | |
| <I/σ(I)> | 38.2 | 3.4 |
| Completeness [%] | 97.7 | 84.4 |
| Redundancy | 9.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M BIS-TRIS propane, 3.5M sodium formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
