3AK1
Superoxide dismutase from Aeropyrum pernix K1, apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-15 |
| Detector | MAC Science DIP-2040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.260, 72.251, 76.652 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 36.130 - 1.570 |
| R-factor | 0.19835 |
| Rwork | 0.196 |
| R-free | 0.23638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wb8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.073 | 0.396 |
| Number of reflections | 103949 | |
| <I/σ(I)> | 23 | 2.9 |
| Completeness [%] | 98.0 | 92.4 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 10% PEG 6000, 8% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
