3AK1
Superoxide dismutase from Aeropyrum pernix K1, apo-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-10-15 |
Detector | MAC Science DIP-2040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.260, 72.251, 76.652 |
Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
Resolution | 36.130 - 1.570 |
R-factor | 0.19835 |
Rwork | 0.196 |
R-free | 0.23638 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wb8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.073 | 0.396 |
Number of reflections | 103949 | |
<I/σ(I)> | 23 | 2.9 |
Completeness [%] | 98.0 | 92.4 |
Redundancy | 4.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 10% PEG 6000, 8% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |