3AGG
X-ray analysis of lysozyme in the absence of Arg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-11 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.609, 78.609, 37.289 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.310 - 1.600 |
R-factor | 0.17091 |
Rwork | 0.169 |
R-free | 0.19828 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a34 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.639 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.072 | 0.072 |
Number of reflections | 15178 | |
<I/σ(I)> | 4.3 | |
Completeness [%] | 99.3 | |
Redundancy | 14.7 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 293 | 0.05M Sodium Acetate, 1.6M Sodium Chloride, pH 4.5, VAPOR DIFFUSION, temperature 293K |