3AF5
The crystal structure of an archaeal CPSF subunit, PH1404 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2822 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.200, 86.200, 238.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.600 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 0.384 |
| Number of reflections | 28592 | |
| <I/σ(I)> | 44.53 | 5.76 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 10.9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1M sodium acetate, 0.1M sodium chloride, 15% (v/v) PEG 6000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
