3AEG

Crystal structure of porcine heart mitochondrial complex II bound with N-Biphenyl-3-yl-2-iodo-benzamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-01
Detector	MAC Science DIP-2040B
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	71.862, 83.874, 294.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 3.270
R-factor	0.248
Rwork	0.244
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zoy
RMSD bond length	0.005
RMSD bond angle	0.910
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.390
High resolution limit [Å]	3.270	3.270
Rmerge	0.096	0.581
Number of reflections	22430
<I/σ(I)>	9.728	1.46
Completeness [%]	78.8	81.6
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	25mM HEPES-NAOH, 7% PEG 4000, 200mM Sucrose, 100mM NaCl, 10mM CaCl2, 0.5mM EDTA, 3% 1,6-haxanediol, 0.5% n-decyl-beta-D-maltoside, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K