3AE7

Crystal structure of porcine heart mitochondrial complex II bound with 2-Iodo-N-(3-isopropoxy-phenyl)-benzamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	71.175, 83.988, 293.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.130 - 3.620
R-factor	0.259
Rwork	0.256
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zoy
RMSD bond length	0.005
RMSD bond angle	0.844
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.149	0.553
Number of reflections	20323
<I/σ(I)>	16.143	2.22
Completeness [%]	97.0	77.8
Redundancy	6.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	25mM HEPES-NAOH, 6% PEG 4000, 200mM Sucrose, 100mM NaCl, 10mM CaCl2, 0.5mM EDTA, 3% 1,6-haxanediol, 0.5% n-decyl-beta-D-maltoside, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K