3ABD
Structure of human REV7 in complex with a human REV3 fragment in a monoclinic crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.870, 50.009, 107.275 |
Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
Resolution | 19.960 - 1.900 |
R-factor | 0.18939 |
Rwork | 0.187 |
R-free | 0.24074 |
Structure solution method | MIR |
RMSD bond length | 0.020 |
RMSD bond angle | 1.689 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | 0.268 |
Number of reflections | 35667 | |
<I/σ(I)> | 14.1 | 3.7 |
Completeness [%] | 97.3 | 83.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 2000 MME, 0.8M sodium formate, 0.1M tris-HCl pH 7.5,, VAPOR DIFFUSION, HANGING DROP, temperature 293K |