3AB2
Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with threonine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.833, 119.093, 124.384 |
| Unit cell angles | 71.85, 69.48, 72.72 |
Refinement procedure
| Resolution | 42.040 - 2.590 |
| R-factor | 0.2344 |
| Rwork | 0.232 |
| R-free | 0.28025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aaw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.150 | |
| Number of reflections | 156726 | |
| <I/σ(I)> | 6.7 | 0.84 |
| Completeness [%] | 98.2 | 96.4 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.2M Ammonium sulfate, 0.05M HEPES-NaOH, 2.1% PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
